fgf19 levels  (Proteintech)

Journal: The FASEB Journal

Article Title: Liver–gut axis signaling regulates circadian energy metabolism in shift workers

doi: 10.1096/fj.202402102R

Figure Lengend Snippet: 24‐h profiles of plasma FGF19 and total BAs in DS versus NS nurses. (A) Plasma FGF19 and (B) total BA levels in DS (Blue) and NS (Orange) nurses. Y‐axis presents means ± SEM; X‐axis presents sample collection time points in average. # indicates statistical significance at p < .05 for the circadian rhythmicity. * or **indicates statistical significance at p < .05 or <0.01 at specified time in/between DS and NS, respectively. Gray and black bars above X‐axis represent the average sleep time of DS and NS nurses, respectively. (C) BA composition in DS and NS nurses over 24 h. BA, bile acid; DS, day shift; NS, night shift.

Article Snippet: Human plasma FGF19 levels were determined by the Human FGF‐19 Quantikine ELISA Kit (R&D Systems) following the manufacture's protocol.

Techniques:

Journal: The FASEB Journal

Article Title: Liver–gut axis signaling regulates circadian energy metabolism in shift workers

doi: 10.1096/fj.202402102R

Figure Lengend Snippet: The cellular circadian rhythm in MCF10A/ PER2‐dLuc reporter cells identified via in vitro bioluminescence assay. (A) Yellow: control; red: 1 μM cambinol; green: 2 μM cambinol; blue: 2 μM cambinol +5 ng/mL recombinant FGF19; brown: 5 ng/mL recombinant FGF19 only. (B) Yellow: control; red: 20 nM EX527; green: 40 nM EX527; blue: 40 nM EX527 + 5 ng/mL recombinant FGF19; brown: 5 ng/mL recombinant FGF19 only. X‐axis, time (days); y‐axis, amplitude.

Article Snippet: Human plasma FGF19 levels were determined by the Human FGF‐19 Quantikine ELISA Kit (R&D Systems) following the manufacture's protocol.

Techniques: In Vitro, ATP Bioluminescent Assay, Control, Recombinant

Journal: American journal of physiology. Gastrointestinal and liver physiology

Article Title: Developmental regulation of the intestinal FGF19 system in domestic pigs.

doi: 10.1152/ajpgi.00312.2017

Figure Lengend Snippet: Fig. 4. Simplified representation of the fibroblast growth factor-19 (FGF19) system and its age-dependent pattern of development in pigs. A: activation of farnesoid X receptor (FXR) in ileal enterocytes by bile acids synthesized by the liver (primary) and gut microbiota (secondary) promotes FGF19 gene transcription and protein secretion into portal circulation (20). Subsequently, FGF19 acts on target cells via interaction with FGF receptors (mainly FGFR4) and the coreceptor -Klotho, which provide to cells their specific responsiveness to FGF19 (17). In liver, FGF19 represses critical enzymes for bile acid synthesis (CYP7A1 and CYP4A21), thereby contributing to a retroinhibitory mechanism of homeostasis for bile acids (20). The action of FGF19 in liver also involves metabolic targets related to protein and glucose metabolism (19). Data reported herein suggest that FGF19 serves regulatory roles, not only in liver (metabolic homeostasis), but also in the intestine (autocrine action) during early life (dashed lines). SHP, small heterodimer partner. B: in pigs, the localization of FGF19 expression in ileum is acquired during lactation, and maximum FGF19 expression and secretion is attained in adulthood. These events correlate with a time-dependent increase in the proportion of primary bile acids that are transformed by gut microbiota into bile acid species that are more potent agonists of FXR. FGF19 receptors also follow an age-dependent pattern of development, showing remarkably high levels of expression in the upper small intestine during the neonatal phase and in liver soon after weaning.

Article Snippet: FGF19 protein levels were quantified in plasma (100 l) using a pig FGF19 ELISA Kit (CSB-E17583p; Cusabio).

Techniques: Activation Assay, Synthesized, Expressing, Transformation Assay